MicroRNA-30c suppressed giant-cell tumor of bone cell metastasis and growth via targeting HOXA1

作者：Ni, L. -Y.; Zhao, J. -D.; Lu, Y. -H.; Li, W.; Li, B. -L.; Wang, X. -C.; Meng, Q. -G.^*

来源：European Review for Medical and Pharmacological Sciences, 2017, 21(21): 4819-4827.

• Giant cell tumor of bone
• miR-30c
• Proliferation and invasion
• HOXA1

OBJECTIVE: To dissect the functioning mode of miR-30c on giant cell tumor of bone cell metastasis and growth and provide therapeutic targets for giant cell tumor of bone. @@@ PATIENTS AND METHODS: By quantitative Real-time polymerase chain reaction (qRT-PCR), miR-30c expression level in 62 pairs of giant cell tumor of bone cells tissue samples and five breast cancer-derived cell lines. Using miR-30c mimics and inhibitors, we analyzed the effects of miR-30c over-expression and knockdown on cell proliferation, invasion, and migration. Dual-luciferase activity assay was recruited to examine the potential target gene HOXA1, which predicted by several databases. Protein level was studied using Western blot. @@@ RESULTS: MiR-30c expressed significantly lower in giant cell tumor of bone tissue samples and cell lines. Over-expression miR-30c in giant cell tumor of bone cells decreased the cell proliferation, invasion, and migration abilities while down-regulation miR-30c in giant cell tumor of bone cells increased these abilities oppositely. Dual-luciferase and Western blot confirmed HOXA1 as a target gene of miR-30c. Furthermore, up-regulation of HOXA1 reserved the suppressive effect of miR-30c over-expression on cell growth and progression. @@@ CONCLUSIONS: miR-30c could suppress giant cell tumor of bone cell proliferation and progression via HOXA1, which might provide a new target for giant cell tumor of bone diagnosis and therapy.

• 出版日期2017-11
• 单位哈尔滨市第一医院; 黑龙江省医院; 哈尔滨医科大学