Nanoparticle-induced photocatalytic head and neck squamous cell carcinoma cell death is associated with autophagy

作者：Hackenberg Stephan; Scherzed Agmal; Gohla Antje; Technau Antje; Froelich Katrin; Ginzkey Christian; Koehler Christian; Burghartz Marc; Hagen Rudolf; Kleinsasser Norbert^*

来源：Nanomedicine, 2014, 9(1): 21-33.

DOI：10.2217/nnm.13.41

• autophagy
• head and neck squamous cell carcinoma
• nanoparticle
• oxidative stress
• photocatalytic
• zinc oxide

Aim: To characterize molecular mechanisms underlying photocatalytic cell death of head and neck squamous cell carcinoma (HNSCC) by zinc oxide nanoparticles (ZnO-NPs). Materials & methods: Human HNSCC-derived FaDu cells were incubated with ZnO-NPs followed by UVA-1 irradiation. Cytotoxicity was assessed by MTT assay and annexin-V propidium iodide test. Autophagy was detected by autophagosome accumulation, conversion of light chain 3 I to II, and lysosomal activity. The generation of reactive oxygen species was measured using the 2,7 '-dichlorofluorescein-diacetate test. Results: Apoptosis-independent cytotoxic effects were induced by 0.2- and 2-mu g/ml ZnO-NPs and UVA-1. FaDu cells promoted autophagosome formation. Significantly elevated light chain 3 II and reactive oxygen species were seen after the combined application of both ZnO-NPs and UVA-1 as photocatalytic treatment. Autophagy probably mediates cell survival under UVA-1 or ZnO-NP exposure alone but induces self-digestive cell death after combined treatment. Conclusion: The effect of autophagy on HNSCC viability after nanoparticle-induced photocatalytic treatment seems to depend on the impact of the physicochemical trigger. Original submitted 14 June 2012; Revised submitted 19 November 2012; Published online 3 June 2013

• 出版日期2014-1