科研之友
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摘要
The western flower thrips, Frankliniella occidentalis, is the most economically important agronomic pest within Thysanoptera because it is both a direct pest of horticulture crops and an efficient vector of plant viruses. Sixty-seven polymorphic SSR loci were identified in the contigs (containing redundant ESTs) generated by assembling 13,839 F. occidentalis ESTs from the public sequence database. Nineteen SSR markers exhibited polymorphism among 860 samples from 43 F. occidentalis populations, with alleles per SSR marker ranging from two to eight, the effective number of alleles (Ne) range from 0.73 to 2.64; the observed (Ho) and expected (He) heterozygosities ranged from 0.09 to 0.77 and 0.12 to 0.96, respectively. The PIC values were from 0.24 to 0.73. AMOVA revealed most genetic variation resided within, rather than between, greenhouse and field isolates. The Mantel test showed no significant differences between genetic and geographical distances. We demonstrated the value of mining the redundant sequences in public sequence databases for the development of polymorphic SSR markers, which can be used for better understanding population variation and spreading of the invasive pest F. occidentalis.
