A High Throughput Soybean Gene Identification System Developed using Soybean Yellow Common Mosaic Virus (SYCMV)

作者：Seo Eun Young; Cho Seunghee; Moon Jae Sun; Gotoh Takafumi; Kim Hong Gi; Domier Leslie L; Lim Seungmo; Kim Kil Hyun; Moon Jung Kyung; Hammond John; Lim Hyoun Sub^*; Song Ki Hak

来源：Journal of the Faculty of Agriculture, Kyushu University, 2015, 60(1): 127-138.

DOI：10.5109/1526307

• Soybean yellow common mosaic virus
• Soybean cDNA library
• Virus induced gene silencing
• Gateway cloning system
• Reverse genetics

Soybean yellow common mosaic virus (SYCMV) was recently reported from Korea, and a subsequent survey of soybean fields found that SYCMV, Soybean yellow mottle mosaic virus (SYMMV), and Soybean mosaic virus (SMV) infections were widespread. SYCMV has recently been developed into a Virus Inducing Gene Silencing (VIGS) vector for use as a reverse genetics tool for soybean, and here we report a modified SYCMV VIGS vector containing a new restriction enzyme site in the 3' non coding region into which we inserted the Gateway system. Ultrasonically generated c.300 bp random fragments of Glycine max cDNA were inserted into the SYCMV VIGS vector, and individual colonies containing G. max cDNA were inoculated to cultivar Williams 82. We monitored the phenotype of inoculated soybean, and selected obvious visible phenotypes caused by SYCMV induced gene silencing which could enable annotation of gene function of unknown gene fragments inserted into SYCMV. Here, we describe development of a high throughput SYCMV VIGS vector for gene function identification in soybean.

• 出版日期2015-2