Molecular cloning, sequencing, and expression analysis of cDNA encoding metalloprotein H (MP II) induced by single and combined metals (Cu(II), Cd(II)) in polychaeta Perinereis aibuhitensis

作者：Yang, Dazuo; Zhou, Yibing^*; Zhao, Huan; Zhou, Xiaoxiao; Sun, Na; Wang, Bin; Yuan, Xiutang

来源：Environmental Toxicology and Pharmacology, 2012, 34(3): 841-848.

DOI：10.1016/j.etap.2012.09.001

• Perinereis aibuhitensis
• Metalloprotein II (MP II)
• Cu(II)
• Cd(II)
• Real-time PCR

We amplified and analyzed the complete cDNA of metalloprotein II (MP II) from the somatic muscle of the polychaete Perinereis aibuhitensis, the full length cDNA is 904 bp encoding 119 amino acids. The MP H cDNA sequence was subjected to BLAST searching in NCBI and was found to share high homology with hemerythrin of other worms. MP II expression of P. aibuhitensis exposed to single and combined metals (Cu(II), Cd(II)) was analyzed using real time-PCR. MP II mRNA expression increased at the start Of Cu(II) exposure, then decreased and finally return to the normal level. Expression pattern of MP H under Cd(II) exposure was time- and dose-dependent. MP II expression induced by a combination of Cd(II) and Cu(II) was similar to that induced by Cd(II) alone.

• 出版日期2012-11
• 单位大连海洋大学; 大连理工大学; 国家海洋环境监测中心