A unique red-emitting two-photon fluorescent probe with tumor-specificity for imaging in living cells and tissues

作者：Kong, Xiuqi; Dong, Baoli; Zhang, Nan; Wang, Chao; Song, Xuezhen; Lin, Weiying^*

来源：Talanta, 2017, 174: 357-364.

DOI：10.1016/j.talanta.2017.06.023

• Red-emitting two-photon fluorescent probe
• High-definition three-dimensional imaging
• Tumor-specific imaging
• In vivo fluorescence imaging

Tumor-specific imaging can provide an attractive approach for the early detection and prognosis of cancer, as well as the precise image-guided tumor-removal surgery. Herein, we describe a unique red-emitting two-photon fluorescent probe (N-BN) for tumor-specific imaging. N-BN utilized Nile Red as the red-emitting two-photon fluorophore and employed biotin as the tumor-specific ligand. In the presence of a variety of biomolecules or in different pH buffers, the fluorescence intensity of N-BN at 655 nm showed no noticeable change. N-BN exhibited the remarkable two-photon absorption cross sections of 15.4 g under excitation at 800 nm. Under the guidance of biotin, N-BN can be applied for the imaging of biotin-receptor positive cancer cells over biotin negative cells under red-emitting one- and two-photon manners. Assisted by high-definition three-dimensional imaging, the living tumor tissues loaded with N-BN could display strong red two-photon fluorescence with a penetration depth of about 90 pm. Moreover, the in vivo and ex vivo imaging studies intuitively revealed that NBN could track the tumor with highly tumor-specific property by a near-infrared manner.

• 出版日期2017-11-1
• 单位济南大学