Albumin Microvascular Leakage in Brains with Diabetes Mellitus

作者：Fujihara Ryuji; Chiba Yoichi; Nakagawa Toshitaka; Nishi Nozomu; Murakami Ryuta; Matsumoto Koichi; Kawauchi Machi; Yamamoto Tetsuji; Ueno Masaki^*

来源：Microscopy Research and Technique, 2016, 79(9): 833-837.

DOI：10.1002/jemt.22708

• albumin
• blood-brain barrier
• diabetes mellitus
• periventricular area

Their aim was to examine whether microvascular leakage of endogenous albumin, a representative marker for blood-brain barrier (BBB) damage, was induced in the periventricular area of diabetic db/db mice because periventricular white matter hyperintensity formation in magnetic resonance images was accelerating in elderly patients with diabetes mellitus. Using light and electron microscopes, and semi-quantitative analysis techniques, immunoreactivity of endogenous albumin, indicating vascular permeability, was examined in the periventricular area and spinal cord of db/db mice and db/+m control mice. Greater immunoreactivity of albumin was observed in the vessel wall of the periventricular area of db/db mice than in controls. Additionally, weak immunoreactivity was observed in the spinal cord of both db/db mice and controls. The number of gold particles, indicating immunoreactivity of albumin, in the perivascular area of db/db mice was significantly higher than that of control mice, but there was no significant difference in the number of particles in the spinal cord between db/db mice and controls. These findings suggest that albumin microvascular leakage, or BBB breakdown, is induced in the periventricular area of diabetic mice. Microsc. Res. Tech. 79:833-837, 2016.

• 出版日期2016-9