Aqueous Extract of Semen Ziziphi Spinosae Exerts Anxiolytic Effects during Nicotine Withdrawal via Improvement of Amygdaloid CRF/CRF1R Signaling

作者：Gu, Changhong; Zhao, ZhengLin; Zhu, Xiaodong; Wu, Tong; Lee, Bong Hyo; Jiao, Yu; Lee, Chul Won; Jung, Dae Hwa; Yang, Chae Ha; Zhao, Rongjie^*; Kim, Sang Chan^*

来源：Evidence-Based Complementary and Alternative Medicine, 2018, 2018: 2419183.

DOI：10.1155/2018/2419183

• CORTICOTROPIN-RELEASING-FACTOR
• ANXIETY-LIKE BEHAVIOR
• CRF
• STRESS
• INVOLVEMENT
• RECEPTORS
• NUCLEUS
• SYSTEMS
• ACTIVATION
• DISORDERS

Anxiety during nicotine withdrawal (NicW) is a key risk factor for smoking relapse. Semen Ziziphi Spinosae (SZS), which is a prototypical hypnotic-sedative herb in Oriental medicine, has been clinically used to treat insomnia and general anxiety disorders for thousands of years. Thus, the present study evaluated the effects of the aqueous extract of SZS (AESZS) on NicW-induced anxiety in male rats that received subcutaneous administrations of nicotine (Nic) (0.4 mg/kg, twice a day) for 7 d followed by 4 d of withdrawal. During NicW, the rats received four intragastric treatments of AESZS (60 mg/kg/d or 180 mg/kg/d). AESZS dose-dependently attenuatedNicW-induced anxiety-like behaviors in the elevated plus maze (EPM) tests and 180 mg/kg/d AESZS inhibited NicW-induced increases in plasma corticosterone. Additionally, the protein and mRNA expressions of corticotropin-releasing factor (CRF) and CRF type 1 receptor (CRF1R) increased in the central nucleus of the amygdala (CeA) during NicW, but these changes were suppressed by 180 mg/kg/d AESZS. A post-AESZS infusion of CRF into the CeA abolished the attenuation of anxiety by AESZS and 180 mg/kg/d AESZS suppressed NicW-induced increases in norepinephrine and 3-methoxy-4-hydroxy-phenylglycol levels in the CeA. The present results suggest that AESZS ameliorated NicW-induced anxiety via improvements in CRF/CRF1R and noradrenergic signaling in the CeA.

• 出版日期2018
• 单位河北医科大学; 牡丹江医学院; 齐齐哈尔医学院