A thiirane linker for isopeptide mimetics by peptide ligation

作者：Kawakami Toru^*; Mishima Yuichi; Kinoshita Misaki; Lee Young Ho; Suetake Isao

来源：Tetrahedron Letters, 2016, 57(19): 2112-2115.

DOI：10.1016/j.tetlet.2016.04.006

• CAE ligation
• Histone
• Post-translational modification
• Thiirane
• Ubiquitin

2-Aminomethylthiirane was used to attach a 1,2-amino-thiol moiety at thiol groups of peptides for use in peptide ligation to produce isopeptide mimetics. To confirm the strategy, a ubiquitinated histone H3 peptide was prepared, and the ubiquitin was correctly folded judging from CD spectra with no influence by the random H3 peptide.

• 出版日期2016-5-11