Musca domestica pupae lectin induces apoptosis in HepG2 cells through a NF-kappa B/p65-mediated caspase pathway

作者：Nie, Jianzeng^*; Cao, Xiaohong; Zhou, Minghui; Zhang, Xinyuan; Zhang, Rongping; Niu, Lili; Xia, Yan; Hou, Lihua; Wang, Chunling

来源：Bulletin du Cancer, 2012, 99(4): E49-E54.

DOI：10.1684/bdc.2012.1557

• Musca domestica pupae lectin
• NF-kappa B/p65
• HepG2
• FLIP
• apoptosis
• caspase

A new lectin (42 kDa) from Musca domestica pupae (MPL) has been known to inhibit proliferation in tumor cells. In this study, flow cytometry analysis showed that MPL induced HepG2 cells apoptosis significantly and the cells were arrested at S phase. MPL inhibited I kappa B-alpha degradation and NF-kappa B/p65 translocation from cytoplasm into nucleus. Simultaneously, the expressions of FLIP, which is a target gene of NF-kappa B/p65 were down-regulated and the caspase-8 and caspase-3 were then activated to induce apoptosis. Taken together, these results showed that MPL induced a caspase-dependent apoptosis via NF-kappa B/p65 pathway in HepG2 cells.

• 出版日期2012-4
• 单位天津科技大学