Laminin and collagen modulate expression of the small leucine-rich proteoglycan fibromodulin in rat anterior pituitary gland

作者：Syaidah Rahimi^*; Horiguchi Kotaro; Fujiwara Ken; Tsukada Takehiro; Kikuchi Motoshi; Yashiro Takashi

来源：Cell and Tissue Research, 2013, 354(2): 633-638.

DOI：10.1007/s00441-013-1698-3

• Anterior pituitary gland
• Fibromodulin
• Folliculostellate cells
• Pericytes
• Small leucine-rich proteoglycans
• Rat

The anterior pituitary is a complex organ consisting of five types of hormone-producing cells, non-hormone-producing cells such as folliculostellate (FS) cells and vascular cells (endothelial cells and pericytes). We have previously shown that FS cells and pericytes produce fibromodulin, a small leucine-rich proteoglycan (SLRP). SLRPs are major proteoglycans of the extracellular matrix (ECM) and are important in regulating cell signaling pathways and ECM assembly. However, the mechanism regulating fibromodulin expression in the anterior pituitary has not been elucidated. Here, we investigate whether fibromodulin expression is modulated by major anterior pituitary ECM components such as laminin and type I collagen. Using transgenic rats expressing green fluorescent protein (GFP) specifically in FS cells, we examine fibromodulin expression in GFP-positive (FS cells) and GFP-negative cells (e.g., pericytes, endocrine cells and endothelial cells). Immunostaining and Western blot analysis were used to assess protein expression in the presence and absence of laminin or type I collagen. We confirmed fibromodulin expression in the pituitary and observed the up-regulation of fibromodulin in FS cells in the presence of ECM components. However, neither laminin nor type I collagen affected expression in GFP-negative cells. This suggests that laminin and type I collagen support the function of FS cells by increasing fibromodulin protein expression in the anterior pituitary.

• 出版日期2013-11