A lectin S-domain receptor kinase mediates lipopolysaccharide sensing in Arabidopsis thaliana

作者：Ranf Stefanie^*; Gisch Nicolas; Schaeffer Milena; Illig Tina; Westphal Lore; Knirel Yuriy A; Sanchez Carballo Patricia M; Zaehringer Ulrich; Hueckelhoven Ralph; Lee Justin; Scheel Dierk

来源：Nature Immunology, 2015, 16(4): 426-433.

DOI：10.1038/ni.3124

The sensing of microbe-associated molecular patterns (MAMPs) triggers innate immunity in animals and plants. Lipopolysaccharide (LPS) from Gram-negative bacteria is a potent MAMP for mammals, with the lipid A moiety activating proinflammatory responses via Toll-like receptor 4 (TLR4). Here we found that the plant Arabidopsis thaliana specifically sensed LPS of Pseudomonas and Xanthomonas. We isolated LPS-insensitive mutants defective in the bulb-type lectin S-domain-1 receptor-like kinase LORE (SD1-29), which were hypersusceptible to infection with Pseudomonas syringae. Targeted chemical degradation of LPS from Pseudomonas species suggested that LORE detected mainly the lipid A moiety of LPS. LORE conferred sensitivity to LPS onto tobacco after transient expression, which demonstrated a key function in LPS sensing and indicated the possibility of engineering resistance to bacteria in crop species.

• 出版日期2015-4