Action potentials recorded from bundles of very thin, gray matter axons in rat cerebellar slices using a grease-gap method

作者：Palani Damodharan; Pekala Dobromila; Baginskas Armantas; Szkudlarek Hanna; Raastad Morten^*

来源：Journal of Neuroscience Methods, 2012, 208(2): 119-127.

DOI：10.1016/j.jneumeth.2012.05.005

• Unmyelinated axons
• Grease-gap
• Membrane potential

We investigated the ability of a grease-gap method to record fast and slow changes of the membrane potential from bundles of gray matter axons. Their membrane potentials are of particular interest because these axons are different from most axons that have been investigated using intra-axonal or gap techniques. One of the main differences is that gray matter axons typically have closely spaced presynaptic specializations, called boutons or varicosities, distributed along their entire paths. %26lt;br%26gt;In response to electrical activation of bundles of parallel fiber axons we were able to record small (128-416 mu V) but stable signals that we show most likely represented a fraction of the trans-membrane action potentials. A less-than 100% fraction prevents measurements of absolute values for membrane potentials, but the good signal-to-noise ratio (typically 10-16) allows detection of changes in resting membrane potential, action potentials and their after-potentials. Because very little is known about the shape of action potentials and after-potentials in these axons we used several independent methods to make it likely that the grease-gap signal was of intra-axonal origin. %26lt;br%26gt;We demonstrate the utility of the method by showing that the action potentials in cerebellar parallel fibers and hippocampal Schaffer collaterals had a slowly decaying, depolarized after-potential. The method is ideal for pharmacological tests, which we demonstrate by showing that the slow after-potential was sensitive to 4-AP, and that the membrane potential was reduced by 200 mu M Ba2+.

• 出版日期2012-7-15