科研之友
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摘要
Background Since influenza virus human isolates grow poorly in embryonated chicken eggs, it is essential to develop high yield seed viruses for large scale production of influenza vaccines. Objectives We aimed to investigate the usefulness of B/Panama/45/90 as a donor to generate high yield B reassortants. B/Brisbane/60/2008, a recommended vaccine virus for 2009/10-2010/11 seasons, was used as a wild type (wt) "target'' strain for potential use as a seed virus for influenza B vaccine production. We also incorporated B/Lee/40 NP gene (a previously known "high yield gene'') into one of the resultant high yield reassortant for further enhancement of the virus yield. Materials and methods B reassortant viruses were prepared by the classical reassortment method. The anti-B/Panama/45/90 HANA antibodies were used for the selection of wt surface antigen carrying viruses. Restriction Fragment Length Polymorphism (RFLP) was used for genotyping of resultant reassortants. Results Reassortants with significantly higher yield than the parental vaccine strain were produced in this reassortment between B/Panama/45/90 and B/Brisbane/60/2008. RT-PCR/RFLP analysis revealed that all the high yield reassortants contained the PB2 of B/Panama/45/90, suggesting that the high yield property of B/Panama/45/90 was donated to the reassortant viruses by PB2. One of the resultant high yield reassortants, NYMC BX-33B, was further reassorted with B/Lee/40 to introduce its NP gene for further enhancement of virus yield. The resultant triple reassortant, NYMC BX-35, has been utilized as a seed virus for influenza B vaccine production for the 2010/11 season. Conclusions A Yamagata lineage strain, B/Panama/45/90, was useful for developing high yield reassortants of B/Brisbane/60/2008. NYMC BX-33, BX-33B, and BX-35 developed in this study were candidate viruses for the 2010/11 Northern Hemisphere vaccine.
- 出版日期2011-5
