Blood-direct InvaderPlus (R) as a new method for genetic testing

作者：Hirasawa Akira^*; Akahane Tomoko; Tanigawara Yusuke; Aoki Daisuke

来源：Personalized Medicine, 2012, 9(6): 657-663.

DOI：10.2217/PME.12.71

• CYP2C9
• genetic testing
• InvaderPlus (R)
• pharmacogenomics
• UGT1A1
• VKORC1

Aim: This study compared the efficiency of Blood-direct InvaderPlus (R) with existing methods for detecting genetic polymorphisms by using clinical samples, and to determine whether this new method can be used for future clinical studies. Materials & methods: We developed a new method, called Blood-direct InvaderPlus, for detecting genetic polymorphisms. Whole-blood samples were subjected to genetic analysis with the new method and also the conventional method. Results: The results obtained with the new method were consistent with those of direct sequencing. Blood-direct InvaderPlus was able to detect gene polymorphisms of UGT1A1*6, *2Z *28, *60, VKORC1 -1639G>A, VKORC1 1173T>C and CYP2C9 *2 and *3 much faster than the conventional methods. Conclusion: Blood-direct InvaderPlus allowed accurate, simple and rapid detection of genetic polymorphisms.

• 出版日期2012-8