Mutations associated with familial Parkinson's disease alter the initiation and amplification steps of alpha-synuclein aggregation

作者：Flagmeier Patrick; Meisl Georg; Vendruscolo Michele; Knowles Tuomas P J; Dobson Christopher M^*; Buell Alexander K^*; Galvagnion Celine^*

来源：Proceedings of the National Academy of Sciences, 2016, 113(37): 10328-10333.

DOI：10.1073/pnas.1604645113

• lipid-induced aggregation
• seeded aggregation
• kinetic analysis
• familial Parkinson's disease
• neurodegenerative disease

Parkinson's disease is a highly debilitating neurodegenerative condition whose pathological hallmark is the presence in nerve cells of proteinacious deposits, known as Lewy bodies, composed primarily of amyloid fibrils of alpha-synuclein. Several missense mutations in the gene encoding alpha-synuclein have been associated with familial variants of Parkinson's disease and have been shown to affect the kinetics of the aggregation of the protein. Using a combination of experimental and theoretical approaches, we present a systematic in vitro study of the influence of disease-associated single-point mutations on the individual processes involved in alpha-synuclein aggregation into amyloid fibrils. We find that lipid-induced fibril production and surface catalyzed fibril amplification are the processes most strongly affected by these mutations and show that familial mutations can induce dramatic changes in the crucial processes thought to be associated with the initiation and spreading of the aggregation of alpha-synuclein.

• 出版日期2016-9-13