Hydrogen sulfide oxidation under anoxic conditions by a nitrate-reducing, sulfide-oxidizing bacterium isolated from the Mae Urn Long Luang hot spring, Thailand

作者：Watsuntorn Wannapawn; Ruangchainikom Chalermchai; Rene Eldon R; Lens Piet N L; Chulalaksananukul Warawut^*

来源：International Biodeterioration & Biodegradation, 2017, 124: 196-205.

DOI：10.1016/j.ibiod.2017.06.013

• Anoxic biodegradation
• Hydrogen sulfide removal
• NR-SOB
• Kinetics

Biological removal of hydrogen sulfide (H2S) has been widely practiced across the world because it overcomes the drawbacks associated with physico-chemical processes. In this study, the role of a novel MAL 1HM19 strain of a nitrate-reducing, sulfide-oxidizing bacterium (NR-SOB) isolated from the Mae Urn Long Luang hot spring (Thailand) was investigated in batch systems for H2S removal under different initial N-NO3- concentrations (60,120 and 240 mg N-NO3-/L) at 35 degrees C. The strain MAL 1 HM19 was capable of removing 100% of the gas-phase H2S within 10 h of incubation. In the liquid-phase, the final products depended on the initial N-NO3- concentration: at 120 and 240 mg N-NOVL, 15% and 18% SO42- formation was detected, while no SO4 was detected at an initial N-NO3- concentration of 60 mg N-NOVL. The identification of strain MAL 1HM19, based on the 16S rDNA nucleotide sequence, revealed it was closely related to Paracoccus sp. (99.93% similarity). The soxB gene was present in strain MAL 1HM19. In addition, strain MAL 1HM19 showed a good capacity to grow at several NaCl concentrations (0.03-7% w/v), at temperatures varying between 20 and 50 degrees C and within the pH range of 7.0-9.0.

• 出版日期2017-10
• 单位UNESCO