A novel allele of the Arabidopsis phytochelatin synthase 1 gene conferring high sensitivity to arsenic and antimony

作者：Kamiya Takehiro^*; Fujiwara Toru

来源：Soil Science and Plant Nutrition, 2011, 57(2): 272-278.

DOI：10.1080/00380768.2011.576398

• Arabidopsis thaliana
• antimony
• arsenic
• phytochelatin

To identify genes involved in arsenite [As(III)] tolerance, we screened for As(III)-sensitive mutants using ethyl methanesulfonate (EMS)-mutagenized seeds of Arabidopsis thaliana. One mutant with high sensitivity to As(III) was isolated from the screening. It had a mutation in Glu52 of phytochelatin synthase 1 (AtPCS1), and introduction of the wild-type AtPCS1 gene rescued the phenotype, confirming that the mutant represented a new allele of atpcs1 (termed as cad1-7). The expression of AtPCS1 from cad1-7 and cad1-3, a second allele of atpcs1, complemented As-sensitive yeast, but not plants. Our study suggests that AtPCS1 activity is differentially regulated between plants and yeast.

• 出版日期2011-4