alpha-Secretase-derived Fragment of Cellular Prion, N1, Protects against Monomeric and Oligomeric Amyloid beta (A beta)-associated Cell Death

作者：Guillot Sestier Marie Victoire; Sunyach Claire; Ferreira Sergio T; Marzolo Maria Paz; Bauer Charlotte; Thevenet Aurelie; Checler Frederic^*

来源：Journal of Biological Chemistry, 2012, 287(7): 5021-5032.

DOI：10.1074/jbc.M111.323626

In physiological conditions, both beta-amyloid precursor protein (beta APP) and cellular prion (PrPc) undergo similar disintegrin-mediated alpha-secretase cleavage yielding N-terminal secreted products referred to as soluble amyloid precursor protein-alpha (sAPP alpha) and N1, respectively. We recently demonstrated that N1 displays neuroprotective properties by reducing p53-dependent cell death both in vitro and in vivo. In this study, we examined the potential of N1 as a neuroprotector against amyloid beta (A beta)-mediated toxicity. We first show that both recombinant sAPP alpha and N1, but not its inactive parent fragment N2, reduce staurosporine-stimulated caspase-3 activation and TUNEL-positive cell death by lowering p53 promoter transactivation and activity in human cells. We demonstrate that N1 also lowers toxicity, cell death, and p53 pathway exacerbation triggered by Swedish mutated beta APP overexpression in human cells. We designed a CHO cell line overexpressing the London mutated beta APP (APP(LDN)) that yields A beta oligomers. N1 protected primary cultured neurons against toxicity and cell death triggered by oligomer-enriched APP(LDN)-derived conditioned medium. Finally, we establish that N1 also protects neurons against oligomers extracted from Alzheimer disease-affected brain tissues. Overall, our data indicate that a cellular prion catabolite could interfere with A beta-associated toxicity and that its production could be seen as a cellular protective mechanism aimed at compensating for an sAPP alpha deficit taking place at the early asymptomatic phase of Alzheimer disease.

• 出版日期2012-2-10
• 单位河北医科大学