Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases

作者：Konno Sho; Ishikawa Fumihiro^*; Suzuki Takehiro; Dohmae Naoshi; Burkart Michael D; Kakeya Hideaki

来源：Chemical Communications, 2015, 51(12): 2262-2265.

DOI：10.1039/c4cc09412c

We describe a general strategy for selective chemical labeling of individual adenylation (A) domains in nonribosomal peptide synthetases (NRPSs) using active site-directed proteomic probes coupled to the 5 '-O-N-(aminoacyl) sulfamoyladenosine (AMS) scaffold with a clickable benzophenone functionality. These proteomic tools can greatly facilitate the molecular identification, functional characterization, and profiling of virtually any kind of A domains of NRPS enzymes in complex biological systems.

• 出版日期2015
• 单位RIKEN