Urinary 1-hydroxypyrene as a comprehensive carcinogenic biomarker of exposure to polycyclic aromatic hydrocarbons: a cross-sectional study of coke oven workers in China

作者：Yamano Yuko^*; Hara Kunio; Ichiba Masayoshi; Hanaoka Tomoyuki; Pan Guowei; Nakadate Toshio

来源：International Archives of Occupational and Environmental Health, 2014, 87(7): 705-713.

DOI：10.1007/s00420-013-0913-6

• Biological monitoring
• Polycyclic aromatic hydrocarbon
• 1-hydroxypyrene
• Urinary metabolite
• Carcinogenesis

Polycyclic aromatic hydrocarbons (PAHs) are multiple compounds that include many carcinogens. We conducted a cross-sectional study in steel plant workers in Anshan, China, to identify biomarkers that reflect the carcinogenicity of PAHs. %26lt;br%26gt;Subjects were 57 workers and 20 controls. Level of personal exposure to PAHs was measured using GC-MS. In accordance with the assessment methods defined by the United States Environmental Protection Agency (US EPA), 15 PAHs were selected for the analysis. For the measurement of urinary metabolites, urine samples were treated with beta-glucuronidase and analyzed using HPLC with a fluorescence detector. %26lt;br%26gt;The mean range of personal exposure to 15 PAHs (total PAHs) was 178.85, 47.08-1,329.45 (geometric mean, 5th and 95th percentile) mu g/m(3). Ten known urinary metabolites (1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 1-hydroxyphenanthrene, 3-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 3-hydroxybenz[a]anthracene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene) and four unknown peaks were detected. The highest correlation was between total PAHs and urinary 2-hydroxynaphthalene (Spearman r = 0.716, P %26lt; 0.01). Among the detected urinary metabolites, 2-hydroxyfluorene, 1-hydroxyphenanthrene, 3-hydroxyphenanthrene, and 1-hydroxypyrene were found to correlate significantly with the %26quot;I pound carcinogenic potency of PAHs%26quot; (sum of seven carcinogenic PAHs calculated from the levels of personal PAHs and relative potency factors), and with the greatest correlation found for 1-hydroxypyrene (Spearman r = 0.630, P %26lt; 0.01). %26lt;br%26gt;The analysis of personal exposure to 15 PAHs and 10 urinary metabolites, and calculation of I pound carcinogenic potency, indicated that urinary 1-hydroxypyrene was the most comprehensive carcinogenic biomarker of exposure to PAHs.

• 出版日期2014-10
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