Novel fluorescent membrane probe 2,3;5,6-bis(cyclohexyl)-BODIPY-labeled phosphatidylcholine

作者：Alekseeva A S; Tretiakova D S; Melnikova D N; Molotkovsky Ul G; Boldyrev I A^*

来源：Russian Journal of Bioorganic Chemistry, 2016, 42(3): 305-309.

DOI：10.1134/S1068162016030031

• fluorescent lipid probes
• BODIPY
• FRET

The novel fluorescent membrane probe, bis(cyclohexyl)-BODIPY (BCHB)-labeled phosphatidylcholine, is structurally similar to 1,3,5,7-tetramethyl-BODIPY (TMB)-labeled phosphatidylcholine. Formally, BCHB and TMB have similar systems of conjugated bonds; however, spectral properties of the probes are notably different. BCHB and TMB have a perfect spectral overlap. The fact makes BCHB a good FRET acceptor for TMB. Thus, the pair of phosphatidylcholines labeled with BCHB and TMB is a good tool for FRET-based membrane studies, e.g. lipid transfer studies.

• 出版日期2016-5