Allelopathic inhibitory effects of Penicillium griseofulvum produced patulin on the seed germination of Orobanche cumana Wallr. and Phelipanche aegyptiaca Pers

作者：Chen, J.; Wei, J.; Gao, J. M.^*; Ye, X. X.; Mcerlean, C. S. P.; Ma, Y. Q.^*

来源：Allelopathy Journal, 2017, 41(1): 65-80.

DOI：10.26651/2017-41-1-1084

• Allelopathy
• inhibitory effect
• Orobanche cumana Wallr.
• patulin
• Penicillium griseofulvum
• Phelipanche aegyptiaca
• seed germination

We studied the effects of identified metabolites and the dry residue from Penicillium griseofulvum (DRPG) on the seed germinations of O. cumana and Phelipanche aegyptiaca Pers. (Orobanche aegyptiaca Pers.). Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) assays were used to isolate and quantify the chemicals in the culture filtrate of P. griseofulvum (CFPG). Results showed that both patulin (a metabolite of P. griseofulvum) and the DRPG solutions at 1.0 mg mL(-1) completely inhibited the seed germination of O. cumana and P. aegyptiaca. The colour and Rf value of patulin were also similar to the fourth band of dry residue of P. griseofulvum in the TLC assay. Moreover, the shape and retention time of patulin peak in the HPLC assay was similar to the main peak of the DRPG. In conclusion, the patulin is the main allelochemical produced from P. griseofulvum, which inhibited the seed germination of O. cumana and P. aegyptiaca.

• 出版日期2017-5
• 单位中国科学院水利部水土保持研究所; 西北农林科技大学