Molecular mapping of a gene conferring resistance to Aphanomyces root rot (black root) in sugar beet (Beta vulgaris L.)

作者：Taguchi Kazunori; Okazaki Kazuyuki; Takahashi Hiroyuki; Kubo Tomohiko; Mikami Tetsuo

来源：Euphytica, 2010, 173(3): 409-418.

DOI：10.1007/s10681-010-0153-8

• Aphanomyces cochlioides
• Sugar beet
• AFLP
• Marker assisted selection
• Resistance
• Linkage analysis

Caused by Aphanomyces cochlioides Drechsler, Aphanomyces root rot is a serious disease of sugar beet (Beta vulgaris L.), for which sources of resistance are scarce. To identify the segregation pattern of the rare resistance trait found in Japanese sugar beet line 'NK-310mm-O', F(1) and BC(1)F(2) seedings, drawn from a cross between 'NK-310mm-O' and susceptible line 'NK-184mm-O', were inoculated with zoospores and their survival evaluated in the greenhouse. Resistance segregation followed was that of a single dominant gene, which was designated Acr1 (Aphanomyces cochlioides resistance 1). Molecular markers tightly linked to Acr1 were identified by bulked segregant analysis of two BC(1)F(2) populations. Fourteen AFLP markers linked to Acr1 were identified, the closest located within +/- 3.3 cM. Three F(5) lines and two BC(2)F(1) lines, selected on the basis of their Acr1-AFLP markers, were tested for their resistance to Aphanomyces root rot in a highly infested field. Results indicated that Acr1 conferred significant resistance to Aphanomyces root rot at the field level. Based on its linkage with CAPS marker tk, a representative marker for chromosome III, Acr1 was located on this chromosome. The clear linkage between tk and Rhizomania resistance trait Rz1, suggests the clustering of major disease resistance genes on chromosome III.

• 出版日期2010-6