Detection of genetically modified plant by electrochemiluminescence PCR method

作者：Liu, JF; Xing, D^*; Shen, XY; Zhu, DB

来源：Progress in Biochemistry and Biophysics, 2004, 31(4): 375-378.

• electrochemiluminescence
• genetically modified plant
• CaMV35S promoter

With the rapid development of the genetically modified plant, more and more genetically modified plant food has been pouring into the market. It has been paid much attention to detection of GMP under the controversy of GMP safety. Electrochemiluminescence method is the chemiluminescent reaction of species that are generated electrochemically at the surface of an electrode. It is a high efficiency and accurate detection method. Electrochemiluminescence PCR method makes electrochemiluminescence technique, PCR technique and two nucleic acid probes hybridization technique band together for the first time. In this method, whether plant containing GM component by detection of CaMV (Cauliflower mosaic virus) 355 promoter are distinguished. The results show thus, it is indicate that, the method can detect genetically modified plant exactly. The method is simple, reliable and correctly for analysis of GMP.

• 出版日期2004-4
• 单位华南师范大学