科研之友
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摘要
Objective: This study aimed to investigate the mRNA expressions of beta-catenin and PTEN in hepatocellular carcinoma exposed to hepatitis B virus (HBV) and aflatoxin B1 (AFB1), and explore the relationship between such expressions under the double exposure and the development of hepatocellular carcinoma. Methods: As per the HBV and AFB1 exposure condition, 108 patients that received surgical excision for hepatocellular carcinoma were divided into 4 groups. Group A: 48 patients, HBV (+)/AFB1 (+); Group B: 27 patients, HBV (+)/AFB1 (-); Group C: 19 patients, HBV (-)/AFB1 (+); Group D: 14 patients, HBV (-)/AFB1 (-). Normal liver tissue (20 specimens) from patients that received surgical excision for hepatic trauma and hemangioma, liver transplant donation etc. was collected at the same time as the control group. RT-PCR was used to determine the expression levels of beta-catenin and PTEN genes, which were then compared between the four groups. Results: As revealed by the RT-PCR examination, there was significant difference in the average semi-quantitative gray value of beta-catenin mRNA between Group A and Group D, as well as Group C and Group D, respectively (1.13 +/- 0.14 vs. 1.01 +/- 0.13, P<0.05; 1.16 +/- 0.18 vs. 1.01 +/- 0.13, P<0.05). Moreover, the differences between Groups A, B, C, D and the control group were all significant (P<0.001). There was also significant difference in the average semi-quantitative gray value of PTEN mRNA between Groups A, B and Groups C, D, respectively (0.54 +/- 0.13 vs. 0.97 +/- 0.16, 0.54 +/- 0.13 vs. 0.92 +/- 0.13, 0.59 +/- 0.16 vs. 0.97 +/- 0.16, 0.59 +/- 0.16 vs. 0.92 +/- 0.13, P<0.05). And the differences between Groups A, B, D and the control group were of statistical significance (P<0.05). Conclusion: The high expression rate of beta-catenin gene may be related to high exposure of AFB1, and the inactivation of PTEN gene is related to the high HBV infection rate.
- 出版日期2016
- 单位广西医科大学; 广西壮族自治区人民医院
