Antibody development to testosterone and its application in capillary electrophoresis-based immunoassay

作者：Chen, Hong-Xu; Zhang, Xin-Xiang^*

来源：Electrophoresis, 2008, 29(16): 3406-3413.

DOI：10.1002/elps.200700660

• abused drugs
• capillary electrophoresis-based immunoassay
• laser-induced fluorescence
• testosterone

The present report describes a rapid, simple, and highly selective approach to perform testosterone competitive immunoassay by CE and LIF detection. The method involves using synthesized fluorescence-labeled testosterone as a tracer to compete with testosterone. Two polyclonal antibodies (antibody (Ab) arised from T-3-BSA (Ab(3)) and Ab arised from T-17-BSA (Ab(17))) and their respective tracers have been optimized and Ab, system is used for the quantification of testosterone by CE-based immunoassay. The method is developed with a wide working range of 3.70-2000 ng/mL and an LOD at 1.11 ng/mL. Tests for normal and positive urine samples show that this method has the potential to be applied in testosterone doping control.

• 出版日期2008-8
• 单位北京大学