科研之友
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摘要
The present work was undertaken to study the immune profile of pre-school going children with Pulmonary Tuberculosis. 40 children visiting the OPD aged between 2-5 years, who were diagnosed as Pulmonary Tuberculosis or Pulmonary plus other site (Disseminated) Tuberculosis. Peripheral venous blood samples were taken and mononuclear cells were harvested. Lymphocyte suspensions were prepared and Lymphocyte proliferation was assessed in vitro after BCG stimulation. The cytokines IFN-gamma, IL-2, and IL-4 in these suspensions were assayed as per Genzyme protocol provided with the kit. The tests were repeated after 3 months and at 6 months at the end of treatment using a standard protocol.
Children with only pulmonary tuberculosis showed a higher lymphocyte proliferation index as compared to children with disseminated disease. The cytokines secreted by cells from patients with a limited and only pulmonary disease showed a significant pattern of a Th1 pattern of cytokine secretion with large amounts of IFN-gamma and IL-2 as compared to children with disseminated disease which responded with a Th2 pattern of cytokine release and mainly IL-4 secretion. Th1 cytokines in only pulmonary tuberculosis were associated with a better outcome. The presence of Th1 pattern of cytokines can be used to predict a better prognosis in pulmonary tuberculosis and a mixed pattern of cytokine secretion that is Th0 in disseminated tuberculosis is also associated with a favorable outcome in pre-school going children.
- 出版日期2011-4
