A New Thiophenyl Pyrazoline Fluorescent Probe for Cu2+ in Aqueous Solution and Imaging in Live Cell

作者:Li, Meng-Meng; Zhao, Wen-Bo; Zhang, Ting-Ting; Fan, Wei-Liu; Xu, Yu; Xiao, Yu; Miao, Jun-Ying; Zhao, Bao-Xiang*
来源:Journal of Fluorescence, 2013, 23(6): 1263-1269.
DOI:10.1007/s10895-013-1259-x

摘要

A new thiophenyl pyrazoline probe for Cu2+ in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu2+ with a detection limit of 1.919 x 10(-7) M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu2+ in living cells and the probe possesses good reversibility.

全文