Development of the Human Fetal Kidney from Mid to Late Gestation in Male and Female Infants

作者:Ryan Danica; Sutherland Megan R; Flores Tracey J; Kent Alison L; Dahlstrom Jane E; Puelles Victor G; Bertram John F; McMahon Andrew P; Little Melissa H; Moore Lynette; Black Mary Jane*
来源:EBioMedicine, 2018, 27: 275-283.
DOI:10.1016/j.ebiom.2017.12.016

摘要

Background: During normal human kidney development, nephrogenesis (the formation of nephrons) is complete by term birth, with the majority of nephrons formed late in gestation. The aim of this study was to morphologically examine nephrogenesis in fetal human kidneys from 20 to 41 weeks of gestation.
Methods: Kidney sampleswere obtained at autopsy from 71 infants that died acutely in utero or within 24 h after birth. Using image analysis, nephrogenic zone width, the number of glomerular generations, renal corpuscle cross-sectional area and the cellular composition of glomeruli were examined. Kidneys from female and male infants were analysed separately.
Findings: The number of glomerular generations formed within the fetal kidneys was directly proportional to gestational age, body weight and kidney weight, with variability between individuals in the ultimate number of generations (8 to 12) and in the timing of the cessation of nephrogenesis (still ongoing at 37 weeks gestation in one infant). There was a slight but significant (r(2) = 0.30, P = 0.001) increase in renal corpuscle cross-sectional area from mid gestation to termin females, but thiswas not evident in males. The proportions of podocytes, endothelial and non-epithelial cells within mature glomeruli were stable throughout gestation.
Interpretation: These findings highlight spatial and temporal variability in nephrogenesis in the developing human kidney, whereas the relative cellular composition of glomeruli does not appear to be influenced by gestational age.

  • 出版日期2018-1