Hyaluronidase decreases neutrophils infiltration to the inflammatory site

作者：Fronza Marcio; Muhr Cornelia; Calheiros da Silveira Denise Sayuri; Sorgi Carlos Arterio; de Paula Rodrigues Stephen Fernandes; Poliselli Farsky Sandra Helena; Garcia Paula Silva Francisco Wanderley; Merfort Irmgard; Faccioli Lucia Helena^*

来源：Inflammation Research, 2016, 65(7): 533-542.

DOI：10.1007/s00011-016-0935-0

• Air pouch
• Cytokines
• Lipid mediators
• Intravital microscopy
• Leukocyte-endothelial interactions
• Neutrophil influx
• Carrageenan
• LPS

Objective To evaluate the in vivo anti-inflammatory potential of bovine hyaluronidase (HYAL) using two different models of acute inflammation.
Methods Air pouches were produced in the dorsal subcutaneous of mice and injected with phosphate saline solution or HYAL. The antiinflammatory action of HYAL was evaluated in carrageenan (Cg)-inflamed air pouches. After 4 and 24 h the cellular influx, protein exudation, cytokines and lipid mediators were evaluated. The action of HYAL on the rolling and adhesion of leukocytes was investigated in the LPS-stimulated mesenteric microcirculation by intravital microscopic.
Results Treatment with HYAL reduced the cellular influx and protein exudation in non-inflamed and inflamed air pouches. HYAL treatment of Cg-inflamed air pouch reduced the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8), leukotriene B4 (LTB4) and LTC4, whereas prostaglandins E-2 (PGE(2)) and D-2 (PGD(2)) concentrations were unchanged. Histological analyses showed that HYAL administration diminished cell infiltration in the air-pouch lining. In LPS-stimulated mesenteric microcirculation, HYAL usage decreased rolling and adhesion of leukocytes, but did not affect the blood vessels diameters.
Conclusion The results demonstrate that HYAL inhibited cellular recruitment, edema formation and pro-inflammatory mediators production, resulting in decreased adherence of leukocytes to blood vessels and tissue infiltration. Our data suggest that HYAL may be considered an effective candidate to ameliorate acute inflammation.

• 出版日期2016-7