Au-doped Fe3O4 nanoparticle immobilized acetylchblinesterase sensor for the detection of organophosphorus pesticide

作者：Min Hong; Qu Yun He; Li Xiao Hua; Xie Zong Hong; Wei Yin Yin; Jin Li Tong^*

来源：Acta Chimica Sinica, 2007, 65(20): 2303-2308.

• Au-Fe3O4
• acetylcholinesterase
• organophosphorus pesticide
• dichlorvos

Au-doped magnetic Fe3O4 nanoparticles were synthesized and used for fabrication of acetylcholinesterase (AChE) immobilized electrochemical sensor for the detection of organophosphorus pesticides (OP), such as dichlorvos. The degree of inhibition of the AChE enzyme by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acetylthiocholine (ATCh). The use of these mixed nanoparticles was shown to provide both significant enzyme stabilization and low detection limit for the biosensor. A value of 10.3 mmol/L for the apparent Michaelis-Menten constant (K-M(app)) was obtained. The biosensor detected as low as 4.0 x 10(-13) mol/L dichlorvos.

• 出版日期2007-10-28
• 单位华东师范大学