Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization

作者：Parisi Antonio^*; Miccolupo Angela; Santagada Gianfranco; Pedarra Carmine; Dambrosio Angela; Normanno Giovanni

来源：Food Control, 2010, 21(5): 770-773.

DOI：10.1016/j.foodcont.2009.09.003

• Colony blot hybridization
• VTEC
• Minced beef
• Milk

Verocytotoxin-producing Escherichia coli (VTEC) are foodborne pathogens that cause Outbreaks linked to Consumption of meat and raw milk. In this note the authors report results obtained from a survey conducted on minced beef and raw bovine milk samples using a Multiplex PCR (M-PCR) for the detection of eae, stx(1), stx(2) and hlyA genes as a screening step followed by a colony blot hybridization (CBH) technique for the isolation of the VTEC. Of 100 minced beef and 123 raw milk samples, 13 (13%) and 7 (5.7%) were positive in the M-PCR and among these 9 and 3 strains were isolated using CBH, respectively. All isolates showed the presence of the stx(2) gene, Single or in association with the other investigated genes. None of the isolates belonged to the 0 157, 026, 091, 0103, 0111 and 0145 serogroups. The Study showed that the use of M-PCR for the screening of samples Coupled with a sensitive and specific detection technique. Could improve the possibility of detection of VTEC strains in foods. Moreover, the presence of VTEC in minced beef and in raw milk confirms their important role as Putative vehicles of infection to humans. Stringent control of these foodstuffs is essential for food safety purposes.

• 出版日期2010-5