AFM characterization of spin-coated multilayered dry lipid films prepared from aqueous vesicle suspensions

作者：Krapf Lisa; Dezi Manuela; Reichstein Werner; Koehler Juergen; Oellerich Silke

来源：Colloids and Surfaces B: Biointerfaces , 2011, 82(1): 25-32.

DOI：10.1016/j.colsurfb.2010.08.006

• AFM
• DPPC
• DOPC
• Spin-coating
• Lipid bilayers
• ITO glass

We present a detailed AFM study on multilayered dry lipid films prepared from aqueous vesicle suspensions. Different preparation techniques were applied in order to optimize the preparation of homogeneous lipid films of various film thicknesses. Suspensions of preformed DOPC/DPPC vesicles were adsorbed onto indium tin oxide-coated glass coverslips. a substrate also commonly employed for the formation of giant liposomes We found that the homogeneity of the lipid films could substantially be improved when applying a spin-coating step during the film preparation. These films were much more homogeneous than those prepared by conventional drop-casting and in addition the film thickness could be controlled. When using a combination of vesicle adsorption and spin-coating the quality and thickness of the films depended crucially on the lipid concentration of the vesicle suspension, the adsorption temperature and the adsorption time. For lipid films prepared by direct sp

• 出版日期2011-1-1