科研之友
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摘要
Gao-Cha is a traditional Chinese health tea made from Acer ginnala. We performed a components and radical scavenging activity analysis to identify any medicinal components in this tea. High performance thin layer chromatography (HPTLC)-1,1-Diphenyl-2-picryhydrazyl (HPTLC-DPPH) assay showed that the methanolic extract contained strong radical scavengers. Quantative analysis revealed that the IC50 of the extract against 1 mM DPPH was 52.7 +/- 0.6 mu g/mL. Bioactive-guided isolations led to procurement of 3 radical scavengers with IC(50)s of 17.5 +/- 2.1, 29.3 +/- 2.5, and 21.6 +/- 1.7 mu g/mL, respectively. Analysis of the high resolution-electric spray ionization-mass spectrometer and 1H, 13C, distortionless enhancement by polarization transfer at 135 degrees, heteronuclear quantum coherence, correlating spectroscopy coupling, and heteronuclear multiple bond coherence (HMBC) data revealed that the compounds were methyl 3, 4, 5-trihydroxybenzoate (1), quercetin-3-O-alpha-rhamnopyranoside (2), and 2,6-bis (3,4,5-trihydroxybenzoyl)-aceritol (3). Bioactive combined components analysis revealed that, apart from compounds 1, 2, and 3, the tea possibly contained radical scavengers: ginnalin A (4) and B (5), 2 '-O-Galloylquercitrin (6) and 3 '-O-Galloyl-quercitrin (7). Compounds 2, 6, and 7 were isolated from Acer ginnala for the first time. The positions of the 2 galloyl moieties in compound 3 were unambiguously established by the HMBC spectrum for the first time.
- 出版日期2010-10
- 单位安徽农业大学
