Microcontact printing of Alzheimer%26apos;s beta-amyloid monomers and fibrils

作者：Huang Danting; Wang Zhibin; Xia Junfei; Zhang Peipei; Kirkland Brett; Paravastu Anant K^*; Guan Jingjiao

来源：European Polymer Journal, 2013, 49(10): 3052-3059.

DOI：10.1016/j.eurpolymj.2013.06.029

• Microcontact printing
• Amyloid beta
• Surface adsorption
• Hydrophobic
• Microparticle
• Microarray

Adsorption of the 40-residue Alzheimer%26apos;s beta-amyloid peptide (A beta 40) on a hydrophobic surface leads to formation of potentially disease-relevant aggregates. Existing techniques are limited in characterizing the adsorbed A beta 40 and producing potentially useful A beta 40 microstructures such as microarrays and microparticles. In this paper, a novel approach based on microcontact printing (mu CP) to studying and utilizing adsorption of A beta 40 monomers and fibril fragments on hydrophobic surface of polydirnethylsiloxane (PDMS) stamps has been developed. By transferring the adsorbed layer from the stamp to a glass substrate, this approach allows easy measurement of thickness of the adsorbed layer. It also enables characterization of the face of the adsorbed layer in contact with the stamp surface. This face exhibits significant higher thioflavin T fluorescence than the face exposed to water, suggesting beta-sheet formation induced by the PDMS surface. The intrinsic stability of the adsorbed layer is evaluated by printing the layer on a water-soluble substrate and exposing it to water vapor or water. Stable particulate microstructures in water are obtained by chemically crosslinking the adsorbed peptides. Moreover, co-micropatterning of the different states of A beta 40 (monomers and fibril fragments) is demonstrated. This mu CP-based approach is simple, versatile, and holds potential for various applications.

• 出版日期2013-10