A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid

作者：Limbut Warakorn; Kanatharana Proespichaya; Mattiasson Bo; Asawatreratanakul Punnee; Thavarungkul Panote^*

来源：Biosensors and Bioelectronics, 2006, 22(2): 233-240.

DOI：10.1016/j.bios.2005.12.025

• thiourea
• capacitive immunosensor
• thioctic acid
• 3-mercaptopropionic acid
• self-assembled monolayer
• alpha-fetoprotein

A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used.

• 出版日期2006-8-15