An arsenic-specific biosensor with genetically engineered Shewanella oneidensis in a bioelectrochemical system

作者：Webster Dylan P; TerAvest Michaela A; Doud Devin F R; Chakravorty Arun; Holmes Eric C; Radens Caleb M; Sureka Swati; Gralnick Jeffrey A; Angenent Largus T^*

来源：Biosensors and Bioelectronics, 2014, 62: 320-324.

DOI：10.1016/j.bios.2014.07.003

• Microbial biosensor
• Bioelectrochemical system (BES)
• Continuous monitoring
• Shewanella
• Synthetic biology
• Arsenic

Genetically engineered microbial biosensors have yet to realize commercial success in environmental applications due, in part, to difficulties associated with transducing and transmitting traditional bioluminescent information. Bioelectrochemical systems (BESs) output a direct electric signal that can be incorporated into devices for remote environmental monitoring. Here, we describe a BES-based biosensor with genetically encoded specificity for a toxic metal. By placing an essential component of the metal reduction (Mtr) pathway of Shewanella oneidensis under the control of an arsenic-sensitive promoter, we have genetically engineered a strain that produces increased current in response to arsenic when inoculated into a BES. Our BES-based biosensor has a detection limit of similar to 40 mu M arsenite with a linear range up to 100 mu M arsenite. Because our transcriptional circuit relies on the activation of a single promoter, similar sensing systems may be developed to detect other analytes by the swap of a single genetic part.

• 出版日期2014-12-15