Proteomic analysis of the eyespot of Chlamydomonas reinhardtii provides novel insights into its components and tactic movements

作者：Schmidt Melanie; Gessner Gunther; Matthias Luff; Heiland Ines; Wagner Volker; Kaminski Marc; Geimer Stefan; Eitzinger Nicole; Reissenweber Tobias; Voytsekh Olga; Fiedler Monika; Mittag Maria; Kreimer Georg^*

来源：Plant Cell, 2006, 18(8): 1908-1930.

DOI：10.1105/tpc.106.041749

Flagellate green algae have developed a visual system, the eyespot apparatus, which allows the cell to phototax. To further understand the molecular organization of the eyespot apparatus and the phototactic movement that is controlled by light and the circadian clock, a detailed understanding of all components of the eyespot apparatus is needed. Wedeveloped a procedure to purify the eyespot apparatus from the green model alga Chlamydomonas reinhardtii. Its proteomic analysis resulted in the identification of 202 different proteins with at least two different peptides ( 984 in total). These data provide new insights into structural components of the eyespot apparatus, photoreceptors, retina(I)- related proteins, members of putative signaling pathways for phototaxis and chemotaxis, and metabolic pathways within an algal visual system. In addition, we have performed a functional analysis of one of the identified putative components of the phototactic signaling pathway, casein kinase 1 (CK1). CK1 is also present in the flagella and thus is a promising candidate for controlling behavioral responses to light. We demonstrate that silencing CK1 by RNA interference reduces its level in both flagella and eyespot. In addition, we show that silencing of CK1 results in severe disturbances in hatching, flagellum formation, and circadian control of phototaxis.

• 出版日期2006-8