Determination of aflatoxin B-1 in alcoholic beverages: comparison of one- and two-photon-induced fluorescence

作者：Rasch Claudia; Boettcher Maike; Kumke Michael^*

来源：Analytical and Bioanalytical Chemistry, 2010, 397(1): 87-92.

DOI：10.1007/s00216-010-3530-1

• Aflatoxin B-1
• Fluorescence
• Two-photon absorption
• Two-photon-absorption cross-section
• Beer
• Wine

The qualitative and quantitative analysis of aflatoxin B-1 in a model system (water/ethanol), in different wines and in beer using one- and two-photon-induced fluorescence is discussed. The absorption and fluorescence properties of aflatoxin B-1 depend on the solvent and pH. The two-photon-absorption cross-section was calculated for aflatoxin B-1 in beer and wine (sigma (2) similar to 25 GM) for excitation at 720 nm. A comparison of the one- and two-photon- induced fluorescence results showed that the disturbance due to background emission originating from matrix constituents is significantly reduced under two-photon-excitation conditions. The limit of detection for the one- and two-photon-induced fluorescence was determined.

• 出版日期2010-5