Non-emissive plastic colour filters for fluorescence detection

作者：Yamazaki M; Krishnadasan S; deMello A J; deMello J C^*

来源：Lab on a Chip, 2012, 12(21): 4313-4320.

DOI：10.1039/c2lc40718c

We report the fabrication of non-emissive short- and long-pass filters on plastic for high sensitivity fluorescence detection. The filters were prepared by overnight immersion of titania-coated polyethylene terephthalate (PET) in an appropriate dye solution - xylene cyanol for short-pass filtering and fluorescein disodium salt for long-pass filtering - followed by repeated washing to remove excess dye. The interface between the titania and the dye molecule induces efficient quenching of photo-generated excitons in the dye molecule, reducing auto-fluorescence to negligible values and so overcoming the principal weakness of conventional colour filters. Using the filters in conjunction with a 505 nm cyan light-emitting diode and a Si photodiode, dose-response measurements were made for T8661 Transfluosphere beads in the concentration range 1 x 10(9) to 1 x 10(5) beads mu L-1, yielding a limit of detection of 3 x 10(4) beads mu L-1. The LED/short-pass filter/T8661/long-pass filter/Siphotodiode combination reported here offers an attractive solution for sensitive, low cost fluorescence detection that is readily applicable to a wide range of bead-based immunodiagnostic assays.

• 出版日期2012