A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa

作者：Ishibashi Yohei; Nagamatsu Yusuke; Miyamoto Tomofumi; Matsunaga Naoyuki; Okino Nozomu; Yamaguchi Kuniko; Ito Makoto^*

来源：Biochemical and Biophysical Research Communications, 2014, 452(4): 873-880.

DOI：10.1016/j.bbrc.2014.08.056

• Chlorophyll
• Digalactosyldiacylglycerol (DGDG)
• Endogalactosylceramidase (EGALC)
• Marine algae
• Phytol
• Transglycosylation

Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Gal alpha/beta 1-6Gal beta 1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Gal alpha 1-6Gal beta 1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.

• 出版日期2014-10-3