F-box Protein Arabidillo-1 Promotes Lateral Root Development by Depressing the Functioning of GA(3) in Arabidopsis

作者：Mu, Changjun; Chen, Ni; Li, Xiaofeng; Jia, Pengfei; Wang, Zhaoyan; Liu, Heng^*

来源：Journal of Plant Biology, 2010, 53(5): 374-380.

DOI：10.1007/s12374-010-9125-8

• Arabidillo-1
• Armadillo
• F-box
• GA
• Lateral roots

In Arabidopsis, Arabidillo-1 and Arabidillo-2 have great sequence homology to Dictyostelium and metazoan beta-catenin/Armadillo, which are important to animal and Dictyostelium development. Arabidillo-1 and Arabidillo-2 promote lateral root formation redundantly in Arabidopsis. Here, we showed that gibberellins (GA(3)) has a greater inhibitory effect on lateral root growth from the null mutant arabidillo-1 than from the wild type, suggesting that the mechanism for Arabidillo-1-regulated modulation of lateral root proliferation is associated with GA(3)-metabolic or signaling pathways. Our yeast two-hybrid analysis demonstrated that Arabidillo-1 interacts with ASK2 and ASK11, and that ASK2 can bind with the F-box domain of Arabidillo-1. Therefore, Arabidillo-1 is involved in the ubiquitin/26S proteasome-mediated proteolytic pathway. Based on these results, we conclude that Arabidillo-1 can degrade some positive regulator of the GA(3) signaling pathway through selective protein degradation of ubiquitin/26S. Moreover, that process is believed to be the mechanism for Arabidillo-1 promotion of lateral root development in Arabidopsis.

• 出版日期2010-10
• 单位兰州大学