Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits

作者：Fang Xinggen; Zhao Rui; Wang Kuizhong; Li Zifu; Yang Penfei; Huang Qinghai; Xu Yi; Hong Bo; Liu Jianmin^*

来源：Journal of Clinical Neuroscience, 2012, 19(9): 1283-1286.

DOI：10.1016/j.jocn.2011.09.038

• Endothelial cells
• Intracranial aneurysm
• Rabbit
• Wound healing

Endothelial progenitor cells (EPC) are believed to be involved in aneurysmal repair and remodeling. The aim of this study was to test this hypothesis and, if true, explore how EPC contribute to aneurysm repair in a rabbit model of elastase-induced carotid aneurysm. Rabbits were divided randomly into an in situ carotid EPC transfusion group (ISCT group, n = 5), and an intravenous EPC transfusion group (IVT group, n = 5). Autologous EPC were double-labeled with Hoechst 33342 and 5,6-carboxyfluorescein diacetate succinimidyl ester before injection into the animals in either the carotid artery (ISCT group) or marginal ear veins (IVT group). Three weeks later, labeled cells in the aneurysms were observed with respect: to location, adhesion, and growth to detect signs of aneurysm repair. Labeled EPC were detected within the neointima in all five aneurysms in the ISCT group and in three of the five aneurysms in the IVT group, but there was no endothelial growth in the aneurysmal neointima in either group. These results show that bone marrow-derived EPC are involved in the process of aneurysm repair in this rabbit model.

• 出版日期2012-9
• 单位中国人民解放军第二军医大学; 皖南医学院