5,6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation

作者：Kumagai Momochika^*; Mishima Takashi; Watanabe Akio; Harada Teppei; Yoshida Izumi; Fujita Kazuhiro; Watai Masatoshi; Tawata Shinkichi; Nishikawa Keisuke; Morimoto Yoshiki

来源：Bioscience Biotechnology and Biochemistry, 2016, 80(7): 1425-1432.

DOI：10.1080/09168451.2016.1153959

• Alpinia zerumbet
• 5
• 6-dehydrokawain
• MC3T3-E1
• osteoblast
• osteoporosis

Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent.

• 出版日期2016-7