Acetylcholinesterase Inhibitors with Photoswitchable Inhibition of beta-Amyloid Aggregation

作者：Chen Xinyu; Wehle Sarah; Kuzmanovic Natascha; Merget Benjamin; Holzgrabe Ulrike; Koenig Burkhard; Sotriffer Christoph A; Decker Michael^*

来源：ACS Chemical Neuroscience, 2014, 5(5): 377-389.

DOI：10.1021/cn500016p

Photochromic cholinesterase inhibitors were obtained from cis-1,2-a-dithienylethene-based compounds by incorporating one or two aminopolymethylene tacrine groups. All target compounds are potent acetyl- (AChE) and butyrylcholinesterase (BChE) inhibitors in the nanomolar concentration range. Compound Jib bearing an octylene linker exhibited interactions with both the catalytic active site (CAS) and the peripheral anionic site (PAS) of AChE. Yet upon irradiation with light, the mechanism of interaction varied from one photochromic form to another, which was investigated by kinetic studies and proved %26quot;photoswitchable%26quot;. The AChE-induced fl-amyloid (Afl) aggregation assay gave further experimental support to this finding: Afil aggregation catalyzed by the PAS of AChE might be inhibited by compound Jib in a concentration-dependent manner and seems to occur only with one photochromic form. Computational docking studies provided potential binding modes of the compound. Docking studies and molecular dynamics (MD) simulations for the ringopen and -closed form indicate a difference in binding. Although both forms can interact with the PAS, more stable interactions are observed for the ring-open form based upon stabilization of a water molecule network within the enzyme, whereas the ringclosed form lacks the required conformational flexibility for an analogous binding mode. The photoswitchable inhibitor identified might serve as valuable molecular tool to investigate the different biological properties of AChE as well as its role in pathogenesis of AD in in vitro assays.

• 出版日期2014-5