A LIMITING FACTOR MEDIATES THE DIFFERENTIAL ACTIVATION OF PROMOTERS BY THE HUMAN PROGESTERONE-RECEPTOR ISOFORMS

作者：MEYER ME; QUIRINSTRICKER C; LEROUGE T; BOCQUEL MT; GRONEMEYER H

来源：Journal of Biological Chemistry, 1992, 267(15): 10882-10887.

The two transcription activation functions (TAFs) of the human progesterone receptor (hPR) have been characterized. TAF-1, located in the N-terminal region A/B, has been narrowed down to a 91-amino acid sequence, which is sufficient for transcription activation in chimeras with the GAL4 DNA binding domain. Both hPR TAF-1 and TAF-2 activate a minimal promoter in yeast. No autonomous TAF could be found in the N-terminal 164 amino acids (designated AB3) which are responsible for the differential activation of promoters by the hPR isoforms A and B. Reduction of the target gene promoter complexity did not alter this differential activation, indicating that AB3 does not require additional promoter-bound factors to exert its effect. Instead, the cell specificity of AB3 and its ability to squelch hPR-induced transcription suggest that this differential isoform activity is due to the effect of a limiting factor which binds to region AB3.

• 出版日期1992-5-25