Analysis of protein interactions with two-hybrid system in cultured insect cells

作者：Mon Hiroaki; Sugahara Ryohei; Hong Sun Mee; Lee Jae Man; Kamachi Yusuke; Kawaguchi Yutaka; Kusakabe Takahiro^*

来源：Analytical Biochemistry, 2009, 392(2): 180-182.

DOI：10.1016/j.ab.2009.05.033

Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, Such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. in this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (12H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the 12H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment.

• 出版日期2009-9-15