Fabrication of NaYF4:Yb,Er Nanoprobes for Cell Imaging Directly by Using the Method of Hydrion Rivalry Aided by Ultrasonic

作者：Li, Zhihua^*; Miao, Haixia; Fu, Ying; Liu, Yuxiang; Zhang, Ran; Tang, Bo^*

来源：Nanoscale Research Letters, 2016, 11(1): 441.

DOI：10.1186/s11671-016-1651-y

• Hydrion rivalry aided by ultrasonic
• NaYF4:Yb,Er
• Bioimaging
• Nanoprobe

A novel method of fabricating water-soluble bio-probes with ultra-small size such as NaYF4:Yb, Er (18 nm), NaGdF4:Yb, Er (8 nm), CaF2:Yb,Er (10 nm), PbS (7 nm), and ZnS (12 nm) has been developed to provide for the solubility switch of nanoparticles from oil-soluble to water-soluble in terms of hydrion rivalry aided by ultrasonic. Using NaYF4: Yb, Er (18 nm) as an example, we evaluate the properties of as-prepared water-soluble nanoparticles (NPs) by using thermogravimetric analyses (TGA), Fourier transform infrared spectroscopy (FTIR), zeta potential (.) testing, and 1H nuclear magnetic resonance ((HNMR)-H-1). The measured. value shows that the newly prepared hydrophilic NaYF4: Yb, Er NPs are the positively charged particles. Acting as reactive electrophilic moiety, the freshly prepared hydrophilic NaYF4:Yb, Er NPs have carried out the coupling with amino acids and fluorescence labeling and imaging of HeLa cells directly. Experiments indicate that the method of hydrion rivalry aided by ultrasonic provides a simple and novel opportunity to transform hydrophobic NPs into hydrophilic NPs with good reactivity, which can be imaging some specific biological targets directly.

• 出版日期2016-10-1
• 单位山东师范大学; 山东大学