Amber mutations in ribosome recycling factors of Escherichia coli and Thermus thermophilus: evidence for C-terminal modulator element

作者：Fujiwara T; Ito K; Nakayashiki T; Nakamura Y^*

来源：FEBS Letters, 1999, 447(2-3): 297-302.

DOI：10.1016/S0014-5793(99)00302-6

• ribosome recycling factor
• Thermus thermophilus ribosome recycling factor
• amber mutation
• intergeneric complementation
• translation termination

Ribosome recycling factor, referred to as RRF, is essential for bacterial growth because of its activity of decomposition of the post-termination complex of the ribosome after release of polypeptides, In this study, we isolated a conditionally lethal amber mutation, named frr-3, in the Escherichia coli RRF gene at amino acid position 161, shelving that the truncation of the C-terminal 25 amino acids of RRF is lethal to E. coli. An RRF gene cloned from Thermus thermophilus, whose protein is 44% identical and 68% similar to E, coli RRF, failed to complement the frr-3(Am) allele, However, truncation of the C-terminal five amino acids conferred intergeneric complementation activity on T, thermophilus RRF, demonstrating the modulator activity of the C-terminal tail.

• 出版日期1999-3-26
• 单位河北医科大学